The Mouse Metabolic Phenotyping Platform at the Division of Endocrinology & Metabolism comprises three components: metabolic chambers, an EchoMRI body-composition analyzer, and physical performance characterization (via treadmill, rotarod, and grip-strength testing).
- Metabolic Chambers (energy expenditure measuring system):
The Columbus Instruments Comprehensive Lab Animal Monitoring System (CLAMS) measures:
- Metabolic performance via indirect calorimetry, O2 consumption and CO2 production, Respiratory Exchange Rate (RER), and Energy Expenditure
- Food intake: both amount and temporal pattern
- Locomotor activity via sensor frames (based on IR beam breaking): total activity along 3 axes or spontaneous wheel running activity
- 8 metabolic chambers are housed within a temperature-controlled cabinet with programmable temperature from 4 to 40 degrees C and programmable light cycles
- EchoMRI system:
The EcoMRI 3-in-1 system provides quantitative information of total body fat and lean mass in awake mice (no anesthesia needed). Composition of organs can also be determined
- Physical performance characterization:
Physical performance of mice can be characterized using:
- Treadmill: Columbus Instruments Exer 6 Open Treadmill with Manual Incline
- Rotarod: Columbus Instruments Rotamex-5
- Grip Strength: Columbus Instruments Grip Strength Meter
General information for experimental design considerations:
A full analysis of respiration in the metabolic chambers requires at least 48 hours (24 hours of acclimatization) over a period of 3 calendar days. To gather statistically significant data, a minimum of 8 mice per group should be analyzed. With 2 groups (one experimental and one control), at least 2 runs are therefore required.
Maximum throughput of the EchoMRI system is approximately 15 mice per hour. Each mouse is scanned twice in rapid succession.